Table of Contents

1. Biological identification; 2. Before DNA; 3. First generation Forensic DNA; 4. STR methods and Loci; 5. DNA analysis and interpretation: Single-source samples and simple mixtures; 6. The curse of sensitivity; 7. From mothers and fathers; 8. Emerging technologies; 9. Emerging issues.

Promotional Information

Using real cases and examples, this book provides an understanding of the science of DNA typing and challenges common misconceptions.

About the Author

Suzanne Bell is Emeritus Professor of Forensic Science at West Virginia University, United States. She served on the National Commission on Forensic Science. In addition to numerous scientific publications and books, she has written multiple editions of Forensic Science: An Introduction to Scientific and Investigative Techniques (5th edition, CRC Press, 2019) and Forensic Chemistry (3rd edition, CRC Press, 2022). John M. Butler is based at the US National Institute of Standards and Technology (NIST). He is one of the most highly cited authors in forensic science and legal medicine and an internationally recognized expert in forensic DNA typing. He is the author of the leading textbooks in forensic DNA typing and served for many years as Associate Editor of Forensic Science International: Genetics.

Reviews

'This is an excellent book, clearly and comprehensively explaining the scientific aspects of DNA and its analysis and the interpretation of DNA profiles used in forensic science. The sections on emerging issues and dispelling common misunderstandings are particularly useful. The book will be an invaluable guide for scientists and non-scientists alike, and the authors are to be congratulated for an outstanding text.' Niamh Nic Daéid, Director of the Leverhulme Research Centre for Forensic Science, University of Dundee, UK

'It is essential that the expectations of the public and legal professionals about forensic DNA technology are not based on crime-fiction TV shows, but rather on reality. The authors of this book explain very clearly what DNA can tell us, address existing misconceptions, and share exciting new developments. I enjoyed the real cases included in the book, all very interesting and informative. Understanding Forensic DNA is definitely a must-read for anyone who wants to become a DNA expert!' Lourdes Prieto, Forensic Science Institute, Santiago de Compostela; General Headquarters of the Spanish Forensic Police, Madrid, Spain

'Understanding Forensic DNA is the perfect resource for anyone wishing to learn about forensic DNA analysis for the very first time. It is a quick read, ideal for the legal professional and for the student who is just beginning to learn about forensic science. Unlike many books of this type, it is written from a historical perspective that outlines how the discipline has evolved in a few short decades. The book thoroughly examines all existing and past technologies in the most comprehensive way and explains the strengths and limitations of each. One would be hard pressed to think of a relevant topic that has been left out. The case examples are instructive and in no way sensational. For someone who teaches forensic science at the college and graduate level, the book provided me with fresh ideas that I can use to improve my teaching of the subject.' Lawrence Quarino, Ph.D., ABC-GKE, Cedar Crest College, Pennsylvania, USA

'This is a go-to book to enhance anyone's understanding of forensic DNA. It is of value to the general public, students of forensic sciences, forensic practitioners, and lawyers. It comprehensively covers all key aspects relating to forensic DNA and plenty of additional intriguing elements you may not have considered. There is a focus on the past, present, and future of forensic DNA with descriptions of key historical events. Complex issues are expertly described with helpful examples, tables, and illustrations. The authors provide a balanced view of the utilization and limitations of forensic DNA analyses, demystify common misunderstandings, and explain current concerns. Reading this book refreshed my understanding and brought new insights to my attention. Another outstanding contribution to forensic science by two exceptional authors.' Roland van Oorschot, Forensic DNA researcher

'Suzanne Bell and John Butler take a journey through the history of forensic genetics. They showcase how this exciting field emerged from its precursor sciences, hematology and serology, and guide the reader through the most recent scientific developments and debates. This entertaining trip provides an unseen density of information and is accessible to a broad audience.' Walther Parson, Institute of Legal Medicine, Medical University of Innsbruck, Austria; PennState University, Pennsylvania, USA

'This book is full of information delivered in a very accessible way. It will be useful for the general public who want to inform themselves about DNA, and also for those scientists who want to be reminded of the historical basis or background of some techniques. It will be an aid to those a little daunted by the more advanced texts. The use of real-life examples throughout the text gives an excellent insight into the uses and limitations of forensic DNA. The basic facts about DNA and the techniques in routine use are understandably described in greater detail than the more modern techniques which are not so mature.' Sheila Willis, Honorary Professor, University of Dundee, Fellow of the Leverhulme Research Centre for Forensic Science, UK; retired Director General, Forensic Science Ireland

'A book that anyone with an interest in DNA profiling will really enjoy. After reading it, you will be well informed, and up to date on current developments. From the inception of biological identification methods to novel methodologies, all is well illustrated by reference to key cases and how the use of DNA profiling has changed forensic investigations. I will be recommending Understanding Forensic DNA as a key text to my undergraduates.' Adrian Linacre, OAM, Chair in Forensic DNA Technologies, Flinders University, Adelaide, Australia

'This book has a very logical structure and touches on all aspects of forensic DNA analysis in an easy-to-understand fashion. Case examples illustrate specific challenges and make the subject matter come alive. I like that the authors include difficult concepts, for example stochastic effects, and relevant calculations, for instance for genotype frequencies and mixture ratios. Forensic DNA analysis is explained but not simplified.' Mechthild Prinz, Ph.D., Professor John Jay College for Criminal Justice, New York, USA

'Forensic DNA analysis contributes significantly to the provision of justice in many jurisdictions around the world. However, there are still misconceptions about what it really is. It is a technical and multidisciplinary subject encompassing biology, statistics, and the law. A book providing a clear and comprehensive description of forensic DNA analysis is very welcome, not only for public awareness but also for improving communication between those involved in this area.' Roberto Puch-Solis, Forensic Statistician, Leverhulme Research Centre for Forensic Science, University of Dundee, UK

'The authors present an excellent overview of the history of forensic DNA analysis, starting with biological identification based on serology in the 1980s and moving on to the first application of DNA profiling by Alec Jeffreys to help solve a case in 1986. The chapters that follow discuss the progressive use of ever smaller fragments and quantities of DNA for identification. This evolution was accomplished by new discoveries about the human genome in conjunction with more sophisticated and sensitive analytical tools and techniques to process DNA. The book discusses the issues that are raised when new methods are applied to DNA evidence, ranging from increased sensitivity to interpretation of data and acceptance in the courtroom. The final chapters deal with cutting-edge technology and new issues, including the use of DNA to create phenotypic profiling, familial searching, genetic genealogy, behavioral profiling, and non-human applications.' Mark Okuda, Evergreen Valley College, San Jose, California, USA

'DNA has brought about a revolution in all fields of the life sciences, and in medicine, biotechnology, and pharmaceuticals. But, arguably, the public knows of DNA from its impact on ancestry and forensics. These are closely related, for in both cases DNA is the key to determining connections, whether between people or between evidence and perpetrator. Bell and Butler's book is therefore welcome and timely, setting out in clear and readable language the basis of DNA-based forensics and how it is used in practice. Most importantly, it also makes clear the limitations of DNA forensics, both the technical and societal issues that it raises. The authors write that their goal is 'to provide an overview of DNA methods, their use, and the current issues in the field.' They have succeeded admirably.' Jan A. Witkowski, Cold Spring Harbor Laboratory, New York, USA

'Understanding Forensic DNA provides an intriguing overview of one of the most innovative fields of applied sciences. Written by two compelling narrators with proven expertise in molecular and forensic genetics, this book not only covers the fascinating scientific background of current forensic DNA technologies but also delves into recent innovations such as investigative genetic genealogy that helped to identify the Golden State Killer. Similarly, other high-profile cases are used to illustrate the evidential power of a given technology. At the same time, a critical distance is maintained, such as when addressing cognitive bias or discussing the use of high-throughput genotyping that may threaten the privacy of suspects or witnesses. This book is worth reading not only for forensic science aficionados but also for DNA experts who want to discover state-of-the-art resources for education and training.' Peter M. Schneider, Institute of Legal Medicine, Cologne, Germany

'Suzanne Bell and John Butler have put together an outstanding text on forensic DNA that the general public can understand. The reader can easily appreciate the impact and limitations of the use of DNA technology through the excellent descriptions and illustrations. This book should be on the shelf of every educator, student, scientist, lawyer, investigator, or anyone working in the criminal justice system who needs to understand the role of DNA in forensic science.' Thomas A. Brettell, Ph.D., Professor of Chemistry, Cedar Crest College, Pennsylvania, USA

'This easy-to-understand book is a guide I will reference again and again. If you want to truly understand how DNA is used in forensic science, this is the book for you!' CeCe Moore, Chief Genetic Genealogist, Parabon