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This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells

Table of Contents

Part A: Techniques






Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
A cell biologist’s guide to high resolution imaging
Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
Green to red photo-convertible mEosFP aided live imaging in plants
Methods for Cell and Particle Tracking
Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
Visualization of TGN-endosome trafficking in mammalian and drosophila cells
Live cell imaging with chemical specificity using dual frequency CARS microscopy
Imaging intracellular protein dynamics by spinning disk confocal microscopy

Part B: Tools





Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
Coiled-coil tag–probe labeling methods for live-cell imaging of membrane receptors
Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
Open Source Tools for Fluorescent Imaging
Nanoparticle PEBBLE sensors in live cells

About the Author

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science.