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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling.

Table of Contents

 

The Biological Chemistry of Hydrogen Peroxide

Reactive Oxygen Species in the Activation of MAP Kinases

Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells

In vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes

Methods for Studying Oxidative Regulation of Protein Kinase C

P66Shc, Mitochondria and the Generation of Reactive Oxygen Species

Detecting Disulfide Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide Dependent Protein Kinases by H2O2

Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation

Activation of Nrf2 by H2O2: De novo Synthesis versus Nuclear Translocation

H2O2 in the Induction of NF-k B-Dependent Selective Gene Expression

Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRa

Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway

Investigating the Role of Reactive Oxygen Species in Regulating Autophagy

H2O2: A Chemoattractant?

Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells

Effects of H2O2 on Insulin Signaling and the Glucose Transport System in Mammalian Skeletal Muscle

Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle

About the Author

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases. Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.