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Now in its second edition, this updated text examines the new advances made in this field since the publication of the first edition twelve years ago. New concepts presented include monolithic columns, bonded stationery phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, retention models for ions, and capillary electromigration techniques. The book also addresses HPLC detectors, LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, polymer HPLC, field-flow fractionation, affinity and ion chromatography. Significant areas of HPLC application are also explored, including chiral pharmaceutical, environmental, food, and forensic analysis. Explanatory figures, tables, and extensive references supplement the text.
Now in its second edition, this updated text examines the new advances made in this field since the publication of the first edition twelve years ago. New concepts presented include monolithic columns, bonded stationery phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, retention models for ions, and capillary electromigration techniques. The book also addresses HPLC detectors, LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, polymer HPLC, field-flow fractionation, affinity and ion chromatography. Significant areas of HPLC application are also explored, including chiral pharmaceutical, environmental, food, and forensic analysis. Explanatory figures, tables, and extensive references supplement the text.
Fundamentals. Monolithic Stationary Phases in HPLC. Bonded Stationary Phases. Micro-HPLC Chromatography. Two-Dimensional Comprehensive Liquid Chromatography. Gradient Elution Mode. Capillary Electromigration Techniques. LC-MS Interfaces: State of the Art and Emerging Techniques. Control and Effects of Temperature in Analytical HPLC. Nonlinear Liquid Chromatography. Displacement Chromatography in the Separation and Characterization of Proteins and Peptides. Field-Flow Fractionation. Affinity Chromatography. Ion Chromatography: Modes for Metal Ions Analysis. Retention Models for Ions in HPLC. Polymer HPLC. Applications. HPLC in Chiral Pharmaceutical.HPLC in Environmental Analysis. HPLC in Food Analysis. HPLC in Forensic Sciences.
Danilo Corradini is research director at the Institute of Chemical Methodologies of the Italian National Research Council (CNR) and a member of the General Scientific Advisory Board of CNR. His involvement in separation science started in 1976 with his research work on chromatography and electrophoresis for his PhD studies in chemistry, which was carried out at Sapienza University of Rome, Italy, under the direction of Michael Lederer, founder and first editor of the Journal of Chromatography. In 1983--1984, he worked with Csaba Horvath, the pioneer of HPLC, at the Department of Chemical Engineering at Yale University, New Haven (USA), where he initiated his first investigations on the HPLC of proteins and peptides. He continued this at the Institute of Chromatography of CNR after he returned to Italy. Currently, he is the head of the chromatography and capillary electrophoresis research unit of the Institute of Chemical Methodologies in Montelibretti, Rome. His research interests are focused on the theoretical and practical aspects of HPLC and capillary electromigration techniques for analytical scale separations of biopolymers, low molecular mass metabolites, and phytochemicals. He has extensively published in international scientific journals, has been chairman and invited speaker in national and international congresses and meetings, and serves on the editorial boards of several journals. He is the chairman of the Interdivisional Group of Separation Science of the Italian Chemical Society and a member of the International Advisory Board of the Mediterranean Separation Science Foundation Research and Training Center in Messina, Italy. In 2009 the Hungarian Separation Science Society assigned him the Csaba Horvath Memorial Award in recognition of his significant contribution to the development, understanding, and propagation of capillary electrophoresis throughout the world and his involvement in the development of separation science in Hungary.
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