1. Construction and Evaluation of Zinc Finger Nucleases

Hiroshi Ochiai and Takashi Yamamoto

2. Updated overview of TALEN construction systems

Tetsushi Sakuma and Takashi Yamamoto

3. CRISPR/Cas9

Izuho Hatada, Sumiyo Morita, and Takuro Horii

4 SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System

Shohei Maruyama, Takashi Kusakabe, Xinyi Zou, Yoshiaki Kobayashi, Yoshimasa Asano, Qingbo S. Wang, and Kumiko Ui-Tei

5. Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design

Qingbo S. Wang, Kumiko Ui-Tei

6. Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA

Takuro Horii and Izuho Hatada

7. Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing.

Manabu Ozawa, Chihiro Emori, and Masahito Ikawa

8. Generation of Knock-in Mouse by Genome Editing

Wataru Fujii

9. Introduction of genetic mutations into mice by Base Editor and Target-AID

Hiroki Sasaguri

10. Genome editing in mouse and rat by electroporation

Takehito Kaneko

11. Generation of floxed mice by sequential electroporation

Takuro Horii, Ryosuke Kobayashi, and Izuho Hatada

12. Efficient detection of flox mice using in vitro Cre recombination

Ryosuke Kobayashi, Takuro Horii, and Izuho Hatada

13. VCre/VloxP and SCre/SloxP as reliable site-specific recombination systems for genome engineering

Manabu Nakayama

14. Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-phase in Mouse Zygotes

Takaya Abe, Ken-ichi Inoue, and Hiroshi Kiyonari

15. Genome editing of murine liver hepatocytes by AAV vector-mediated expression of Cas9 in vivo

Yuji Kashiwakura and Tsukasa Ohmori

16. Non-Viral Ex Vivo Genome-Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9

Hiromasa Hara1, Natsagdorj Munkh-Erdene, Suvd Byambaa, and Yutaka Hanazono

17. Genome editing of rat

Takehito Kaneko

18. A simple and efficient method for generating KO rats using in vitro fertilized oocytes

Kohtaro Morita, Arata Honda, and Masahide Asano

19. Editing the Genome of the Golden Hamster (Mesocricetus auratus)

Michiko Hirose, Toshiko Tomishima, and Atsuo Ogura

20. Gene targeting in rabbits: single-step generation of knockout rabbits by microinjection of CRISPR/Cas9 plasmids

Arata Honda

21. Genome Editing of Pig

Masahito Watanabe and Hiroshi Nagashima

22. GEEP method: an optimized electroporation-mediated gene-editing approach for establishment of knockout pig lines

Fuminori Tanihara, Maki Hirata, and Takeshige Otoi

23. Genome editing mediated by primordial germ cell in chicken

Jae Yong Han and Hong Jo Lee

24. CRISPR-Cas9-mediated genome modifications in zebrafish

Yusuke Kamachi and Atsuo Kawahara

25. Genome Editing of Medaka

Rie Hara, Satoshi Ansai, and Masato Kinoshita

26. CRISPR-Cas9 based functional analysis in amphibians; Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl

Miyuki Suzuki, Midori Iida, Toshinori Hayashi, and Ken-ichi T Suzuki

27. Genome Editing of Silkworms

Takuya Tsubota, Hiroki Sakai, and Hideki Sezutsu

28. Improved genome editing in the ascidian Ciona with CRISPR/Cas9 and TALEN

Yasunori Sasakura and Takeo Horie

29. Genome editing of C. elegans

Takuma Sugi

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