Hardback : £111.00
Essential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine. * Allows the reader to develop the necessary skills to properly evaluate research articles * Coverage of over 30 commonly-used techniques in the biomedical sciences * Global approach and application, with contributions from leading experts in diverse fields
List of contributors ix Foreword xi Preface xiii Acknowledgements xvii Introduction xix Section A - Basic principles 1 1 Philosophy of science 3 James Ladyman 2 Ingredients of experimental design 9 Nick Colegrave 3 Statistics: a journey that needs a guide 17 Gordon Drummond Section B - Cell and molecular 27 4 Organ bath pharmacology 29 Emma Robinson 5 Small vessel myography 39 Tomoko Kamishima and John M Quayle 6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49 Scott H Randell 7 Electron microscopy (TEM and SEM) 59 Paul Verkade 8 Fluorescence microscopy 67 Mark Jepson 9 Intracellular 'sharp' microelectrode recording 77 Helena C Parkington and Harold A Coleman 10 Single electrode voltage-clamp (SEVC) 85 Harold A Coleman and Helena C Parkington 11 Patch clamp recording 95 Neil Bannister and Phil Langton 12 Production of antibodies 105 Elek Molnar 13 Immunocytochemistry and immunohistochemistry 117 Elek Molnar 14 Immunoprecipitation (IP) 129 David Bates 15 Immunoblotting (western) 137 Samantha F. Moore, Joshua S. Savage and Ingeborg Hers 16 Applications of green fluorescent protein (GFP) 147 Mark Jepson 17 Fluorescent measurement of ion activity in cells 153 Helen Kennedy 18 Detection of exocytosis - real time 163 Anja Teschemacher 19 Viral vector transgenesis 173 Anja Teschemacher 20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179 Lucy F. Donaldson 21 In situ hybridisation (ISH) 187 Lucy F. Donaldson 22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193 Allison Fulford 23 Transcriptome analysis: microarrays 203 Charles Hindmarch 24 Experimental proteomics 215 Thierry Le Bihan Section C - In vivo / integrative 229 25 Behavioural methodology 231 Emma Robinson 26 Genetically modified mouse models 241 Nina Balthasar 27 Wireless recording of cardiovascular signals 247 Julian FR Paton and Fiona D McBryde 28 Electrical stimulation methods 253 Jon Wakerley 29 Extracellular recording 261 Jon Wakerley 30 Antidromic identification 271 Jon Wakerley 31 Event-triggered averaging, including spike-triggered averaging 279 Richard Apps 32 Axonal transport tracing of CNS pathways 285 John Crabtree 33 Cardiovascular methods: general considerations for human studies 291 Erica A Wehrwein and Michael J Joyner 34 Measuring cardiac output in humans 299 Erica A Wehrwein and Michael J Joyner 35 Measuring peripheral blood flow in humans 311 Erica A Wehrwein and Michael J Joyner Index 319
Show moreEssential Guide to Reading Biomedical Papers: Recognising and Interpreting Best Practice is an indispensable companion to the biomedical literature. This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. This unique book will be an invaluable resource for students, technicians and researchers in all areas of biomedicine. * Allows the reader to develop the necessary skills to properly evaluate research articles * Coverage of over 30 commonly-used techniques in the biomedical sciences * Global approach and application, with contributions from leading experts in diverse fields
List of contributors ix Foreword xi Preface xiii Acknowledgements xvii Introduction xix Section A - Basic principles 1 1 Philosophy of science 3 James Ladyman 2 Ingredients of experimental design 9 Nick Colegrave 3 Statistics: a journey that needs a guide 17 Gordon Drummond Section B - Cell and molecular 27 4 Organ bath pharmacology 29 Emma Robinson 5 Small vessel myography 39 Tomoko Kamishima and John M Quayle 6 Mammalian cell cultures: the example of airway epithelial cell cultures for cystic fibrosis research 49 Scott H Randell 7 Electron microscopy (TEM and SEM) 59 Paul Verkade 8 Fluorescence microscopy 67 Mark Jepson 9 Intracellular 'sharp' microelectrode recording 77 Helena C Parkington and Harold A Coleman 10 Single electrode voltage-clamp (SEVC) 85 Harold A Coleman and Helena C Parkington 11 Patch clamp recording 95 Neil Bannister and Phil Langton 12 Production of antibodies 105 Elek Molnar 13 Immunocytochemistry and immunohistochemistry 117 Elek Molnar 14 Immunoprecipitation (IP) 129 David Bates 15 Immunoblotting (western) 137 Samantha F. Moore, Joshua S. Savage and Ingeborg Hers 16 Applications of green fluorescent protein (GFP) 147 Mark Jepson 17 Fluorescent measurement of ion activity in cells 153 Helen Kennedy 18 Detection of exocytosis - real time 163 Anja Teschemacher 19 Viral vector transgenesis 173 Anja Teschemacher 20 Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR 179 Lucy F. Donaldson 21 In situ hybridisation (ISH) 187 Lucy F. Donaldson 22 Methods of interference (antisense, siRNAs and dominant negative mutations) 193 Allison Fulford 23 Transcriptome analysis: microarrays 203 Charles Hindmarch 24 Experimental proteomics 215 Thierry Le Bihan Section C - In vivo / integrative 229 25 Behavioural methodology 231 Emma Robinson 26 Genetically modified mouse models 241 Nina Balthasar 27 Wireless recording of cardiovascular signals 247 Julian FR Paton and Fiona D McBryde 28 Electrical stimulation methods 253 Jon Wakerley 29 Extracellular recording 261 Jon Wakerley 30 Antidromic identification 271 Jon Wakerley 31 Event-triggered averaging, including spike-triggered averaging 279 Richard Apps 32 Axonal transport tracing of CNS pathways 285 John Crabtree 33 Cardiovascular methods: general considerations for human studies 291 Erica A Wehrwein and Michael J Joyner 34 Measuring cardiac output in humans 299 Erica A Wehrwein and Michael J Joyner 35 Measuring peripheral blood flow in humans 311 Erica A Wehrwein and Michael J Joyner Index 319
Show moreList of contributors ix
Foreword xi
Preface xiii
Acknowledgements xvii
Introduction xix
Section A - Basic principles 1
1 Philosophy of science 3
James Ladyman
2 Ingredients of experimental design 9
Nick Colegrave
3 Statistics: a journey that needs a guide 17
Gordon Drummond
Section B - Cell and molecular 27
4 Organ bath pharmacology 29
Emma Robinson
5 Small vessel myography 39
Tomoko Kamishima and John M Quayle
6 Mammalian cell cultures: the example of airway epithelial cell
cultures for cystic fibrosis research 49
Scott H Randell
7 Electron microscopy (TEM and SEM) 59
Paul Verkade
8 Fluorescence microscopy 67
Mark Jepson
9 Intracellular 'sharp' microelectrode recording 77
Helena C Parkington and Harold A Coleman
10 Single electrode voltage-clamp (SEVC) 85
Harold A Coleman and Helena C Parkington
11 Patch clamp recording 95
Neil Bannister and Phil Langton
12 Production of antibodies 105
Elek Molnar
13 Immunocytochemistry and immunohistochemistry 117
Elek Molnar
14 Immunoprecipitation (IP) 129
David Bates
15 Immunoblotting (western) 137
Samantha F. Moore, Joshua S. Savage and Ingeborg Hers
16 Applications of green fluorescent protein (GFP) 147
Mark Jepson
17 Fluorescent measurement of ion activity in cells 153
Helen Kennedy
18 Detection of exocytosis – real time 163
Anja Teschemacher
19 Viral vector transgenesis 173
Anja Teschemacher
20 Polymerase chain reaction (PCR) and reverse transcription
(RT)-PCR 179
Lucy F. Donaldson
21 In situ hybridisation (ISH) 187
Lucy F. Donaldson
22 Methods of interference (antisense, siRNAs and dominant
negative mutations) 193
Allison Fulford
23 Transcriptome analysis: microarrays 203
Charles Hindmarch
24 Experimental proteomics 215
Thierry Le Bihan
Section C - In vivo / integrative 229
25 Behavioural methodology 231
Emma Robinson
26 Genetically modified mouse models 241
Nina Balthasar
27 Wireless recording of cardiovascular signals 247
Julian FR Paton and Fiona D McBryde
28 Electrical stimulation methods 253
Jon Wakerley
29 Extracellular recording 261
Jon Wakerley
30 Antidromic identification 271
Jon Wakerley
31 Event-triggered averaging, including spike-triggered
averaging 279
Richard Apps
32 Axonal transport tracing of CNS pathways 285
John Crabtree
33 Cardiovascular methods: general considerations for human
studies 291
Erica A Wehrwein and Michael J Joyner
34 Measuring cardiac output in humans 299
Erica A Wehrwein and Michael J Joyner
35 Measuring peripheral blood flow in humans 311
Erica A Wehrwein and Michael J Joyner
Index 319
Dr. Philip David Langton, Senior Lecturer and Academic
Director of E-Learning, Department of Physiology, University of
Bristol
Dr Philip Langton is currently a Senior Lecturer and Academic
Director of e-Learning in the Faculty of Medical and Veterinary
Sciences at the University of Bristol, UK.?Dr Langton is an
advocate for excellence in all forms of learning, teaching and
assessment in HE. He is an enthusiastic undergraduate and graduate
teacher, contributing to Medical Sciences and Physiological
Sciences programmes. Finalist of 'Bioscience Teacher of the Year'
for 2011, Dr Langton is passionate about providing an excellent
learning experience for students in ways that are stimulating and
rewarding for University staff, and disseminating innovative and
high-quality learning practices.
The book would be of most benefit to new researchers orearly career scientists though with some benefits to the moreexperienced scientist moving into a new discipline. (Microbiology Today, 1 September 2013) As a guide to how to properly ask questions of life andhow to interpret its often-enigmatic answers, this book is a mustread. Summing Up: Highly recommended. Upper-divisionundergraduates, graduate students, researchers/faculty, andprofessionals. (Choice, 1 August 2013) Too many recent bioscience graduates lack significantresearch lab experience. Even research projects can expose them tojust a few techniques. Appraisal of research papers inundergraduate courses tends to address the results, but rarelyincludes a critical evaluation of the researchers methodology. Inreality, few bioscientists can claim a working knowledge of morethan a handful of the techniques covered in this collection. These shortcomings can be greatly overcome thanks to this book.The contributors are active research scientists of high quality.Each addresses the methods in a critical sense and provide anexpert's view of the advantages and pitfalls. There is noequivalent book currently available. Dr David J. Miller onbehalf of The Physiological Society Research questions require the scientist to employmolecular, cell, organism and population approaches, and the readerof scientific literature to carefully consume data from each ofthose environments. This book provides a clear and concisesummary of the research techniques and approaches that generatedata. Importantly, the authors identify appropriate uses andcautionary caveats, essential for readers who do not use thetechnique. This book is essential for accurate and carefulinterpretation of scientific literature. Professor Robert G.Carroll, Editor Advances in Physiology Education
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