Table of Contents

1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET

2: Evaluating functional ligand-GPCR interactions in cell-based assays

3: Assays for detecting arrestin interaction with GPCRs

4: BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation

5: Cannabinoid Receptor CB1 and CB2 Interacting Proteins: Techniques, Progress and Perspectives

6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization

7: Nanobodies as Sensors of GPCR Activation and Signaling

8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells

9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology

10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells

11: Methods for Binding Analysis of Small GTP-Binding Proteins with their Effectors

12: Protein-protein interactions in the brain: protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho- proteomics analysis

13: Protein-Protein Interactions at a glance: protocols for the visualization of biomolecular interactions

14: Interactions between Noncoding RNAs as Epigenetic Regulatory Mechanisms in Cardiovascular Diseases

About the Author

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.